Anti-VPS28 (171-185) antibody produced in rabbit

Name	Anti-VPS28 (171-185) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	V3140
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 171-185 of human VPS28
Description	Rabbit Polyclonal
Gene	VPS28
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-VPS28 (171-185): <B>Cat. No. V3140</B>: Immunofluorescence of HUVEC cells using VPS28 (171-185), Cat. No. V3140 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-VPS28 (171-185): Cat. No. V3140: Immunofluorescence of HUVEC cells using VPS28 (171-185), Cat. No. V3140 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human VPS28 was separated on SDS-PAGE, blotted with Anti-VPS28 (171-185) (<B>Cat. No. V3140</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500 <BR/>2. Antibody dilution 1:500 + VPS28 immunizing peptide (human, 171-185)<BR/>3. Antibody dilution 1:1,000

Immunoblotting
Lysate of HEK-293T cells overexpressing human VPS28 was separated on SDS-PAGE, blotted with Anti-VPS28 (171-185) (Cat. No. V3140) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + VPS28 immunizing peptide (human, 171-185)
3. Antibody dilution 1:1,000

<B>Chart</B><BR/>Anti-VPS28 (171-185): <B>Cat. No. V3140</B>: Intensity analysis of Anti-VPS28 (171-185) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-VPS28 (171-185): Cat. No. V3140: Intensity analysis of Anti-VPS28 (171-185) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.