Western blot analysis of anti-PIP5K1B Pab (Cat. #AP8038a) in human placenta cell lysate. PIP5K1B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human brain tissue reacted with PIP5K1B antibody (N-term) (Cat.# AP8038a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes : Anti-PIP5K1B Antibody (M1) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.