SIX5 Antibody (Center) (Cat. #AP12740c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Fluorescent confocal image of Hela cell stained with SIX5 Antibody (Center)(Cat#AP12740c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SIX5 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SIX5 immunoreactivity is localized to Cytoplasm significantly.
SIX5 Antibody (Center) (Cat. #AP12740c) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SIX5 antibody detected the SIX5 protein (arrow).