Anti-14-3-3 antibody
| Name | Anti-14-3-3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab6081 |
| Prices | $379.00 |
| Sizes | 1 ml |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide with the sequence DKSELVQKAKLAEQAERYD , mapping to the amino terminal domain of human 14-3-3 |
| Description | Rabbit Polyclonal |
| Gene | YWHAB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-14-3-3 antibody (ab6081) at 1/200 dilutionLane 1 : Spinal Cord (Mouse) Tissue Lysate Lane 2 : Lung (Rat) Tissue Lysate Lane 3 : Lung (Mouse) Whole Cell Lysate - normal tissue (ab29297)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
ICC/IF image of ab6081 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6081, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Scaffold functions of 14-3-3 adaptors in B cell immunoglobulin class switch DNA - Scaffold functions of 14-3-3 adaptors in B cell immunoglobulin class switch DNA
Lam T, Thomas LM, White CA, Li G, Pone EJ, Xu Z, Casali P. PLoS One. 2013 Nov 25;8(11):e80414.
Oxidative damage of 14-3-3 zeta and gamma isoforms in Alzheimer's disease and - Oxidative damage of 14-3-3 zeta and gamma isoforms in Alzheimer's disease and
Santpere G, Puig B, Ferrer I. Neuroscience. 2007 Jun 8;146(4):1640-51. Epub 2007 Apr 19.