Anti-14-3-3 epsilon antibody [EPR3918]
| Name | Anti-14-3-3 epsilon antibody [EPR3918] |
|---|---|
| Supplier | Abcam |
| Catalog | ab92311 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3918 |
| Applications | ICC/IF ICC/IF WB FC IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues in Human 14-3-3 epsilon (UniProt ID: P62258) |
| Description | Rabbit Monoclonal |
| Gene | YWHAE |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing SH-SY5Y cells stained with ab92311 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92311, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-14-3-3 epsilon antibody [EPR3918] (ab92311) at 1/1000 dilutionLane 1 : Human fetal brain cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : 293T cell lysateLane 4 : SW480 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/145_14-3-3-epsilon-Primary-antibodies-ab92311-1.jpg)
All lanes : Anti-14-3-3 epsilon antibody [EPR3918] (ab92311) at 1/1000 dilutionLane 1 : Human fetal brain cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : 293T cell lysateLane 4 : SW480 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution
ab92311 at 1/100 dilution staining 14-3-3 epsilon in HeLa cells by Immunofluorescence.
Product References
Neuroprotective effect of TAT-14-3-3epsilon fusion protein against cerebral - Neuroprotective effect of TAT-14-3-3epsilon fusion protein against cerebral
Zhu Y, Bu Q, Liu X, Hu W, Wang Y. PLoS One. 2014 Mar 26;9(3):e93334.
Functional EF-hands in neuronal calcium sensor GCAP2 determine its - Functional EF-hands in neuronal calcium sensor GCAP2 determine its
Hoyo NL, Lopez-Begines S, Rosa JL, Chen J, Mendez A. PLoS Genet. 2014 Jul 24;10(7):e1004480.
Inactivation of the Hippo tumour suppressor pathway by integrin-linked kinase. - Inactivation of the Hippo tumour suppressor pathway by integrin-linked kinase.
Serrano I, McDonald PC, Lock F, Muller WJ, Dedhar S. Nat Commun. 2013;4:2976.