YWHAE

Gene Symbol YWHAE
Entrez Gene 7531
Alt Symbol 14-3-3E, HEL2, KCIP-1, MDCR, MDS
Species Human
Gene Type protein-coding
Description tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
Other Description 14-3-3 epsilon|14-3-3 protein epsilon|epididymis luminal protein 2|mitochondrial import stimulation factor L subunit|protein kinase C inhibitor protein-1|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide|tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
Swissprots D3DTH5 B3KY71 P62258 Q7M4R4 Q4VJB6 Q53XZ5
Accessions BAA32538 EAW90627 EAW90628 EAW90629 P62258 AK128785 BAG54733 AK295260 BAG58249 AK295997 BAG58772 AK296050 BAG58814 AK296555 BAG59178 AK316185 BAH14556 AK316201 BAH14572 AY883089 AAX68683 BC000179 AAH00179 BC001440 AAH01440 BM755263 BT007161 AAP35825 DC365395 EU176525 ABW03326 EU794581 ACJ13635 U20972 AAC50175 U28936 AAA75301 U43399 AAC50625 U43430 AAD00026 U54778 AAC50710 XM_005256784 XP_005256841 XM_006725298 XP_006725361 NM_006761 NP_006752 NR_024058
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Subcellular Location Cytoplasm {ECO:0000250}. Melanosome {ECO:0000269|PubMed:12042314, ECO:0000269|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Top Pathways Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis, Viral carcinogenesis, PI3K-Akt signaling pathway

14-3-3 ε Antibody - 9635 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using 14-3-3 ε Antibody.

14-3-3 ε (A-14) - sc-31961 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
14-3-3 ε (A-14): sc-31961. Western blot analysis of 14-3-3 ε expression in SW480 (A), NIH/3T3 (B), Jurkat (C), IMR-32 (D) and Daudi (E) whole cell lysates.

14-3-3 ε (E-20) - sc-31962 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
14-3-3 ε (E-20): sc-31962. Western blot analysis of 14-3-3 ε expression in non-transfected 293T: sc-117752 (A), human 14-3-3 ε transfected 293T: sc-175743 (B) and SW480 (C) whole cell lysates.

14-3-3 ε (T-16) - sc-1020 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
14-3-3 ε (T-16): sc-1020. Western blot analysis of 14-3-3 ε expression in KNRK whole cell lysates.

14-3-3 ε (8C3) - sc-23957 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
14-3-3 ε (8C3): sc-23957. Western blot analysis of 14-3-3 ε expression in KNRK (A), SW480 (B), T98G (C), EOC 20 (D) and Caco-2 (E) whole cell lysates.

14-3-3 ε (12) - sc-135816 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
14-3-3 ε (12): sc-135816. Western blot analysis of 14-3-3 ε expression in HeLa whole cell lysate.

14-3-3 ε (5A5) - sc-130547 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
14-3-3 ε (5A5): sc-130547. Western blot analysis of 14-3-3 ε expression in mouse brain extract.

Anti-14-3-3 epsilon antibody - ab196891 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-14-3-3 epsilon antibody (ab196891) at 1/500 dilutionLane 1 : T47D cell extractLane 2 : Jurkat cell extractLane 3 : PC12 cell extractLane 4 : COS1 cell extractLane 5 : COS7 cell extractLane 6 : NIH 3T3 cell extractLane 7 : SW480 cell extract

Anti-14-3-3 epsilon antibody - ab190742 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-14-3-3 epsilon antibody (ab190742) at 1/500 dilution + extracts from A375 cells

Anti-14-3-3 epsilon antibody - ab180836 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-14-3-3 epsilon antibody (ab180836) at 1/500 dilutionLane 1 : T47D cell lysateLane 2 : Jurkat cell lysateLane 3 : PC12 cell lysateLane 4 : COS1 cell lysateLane 5 : COS7 cell lysateLane 6 : NIH3T3 cell lysateLane 7 : SW480 cell lysate

Anti-14-3-3 epsilon antibody - ab64983 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-14-3-3 epsilon antibody (ab64983) at 1/500 dilutionLane 1 : Extracts from HepG2 cellsLane 2 : Extracts from Jurkat cellsLane 3 : Extracts from Jurkat cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-14-3-3 epsilon antibody - ab199651 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-14-3-3 epsilon antibody (ab199651) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell line lysateLysates/proteins at 35 µg per lane.SecondaryGoat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

Anti-14-3-3 epsilon antibody [5A5] - ab71506 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5A5
Anti-14-3-3 epsilon antibody [5A5] (ab71506) at 1/1000 dilution + Mouse brain extract at 50 µgSecondaryGoat anti-mouse secondary conjugated to HRPdeveloped using the ECL technique

Anti-14-3-3 epsilon antibody [EPR3918] - ab92311 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3918
Overlay histogram showing SH-SY5Y cells stained with ab92311 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92311, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-14-3-3 epsilon antibody [EPR3919] - ab108210 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EPR3919
All lanes : Anti-14-3-3 epsilon antibody [EPR3919] (ab108210)Lane 1 : Fetal brain lysateLane 2 : SW480 cell lysateLane 3 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-YWHAE / 14-3-3 Epsilon Antibody (aa1-255, clone 5A5) IHC-plus™ - LS-B2948 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 5A5
Anti-YWHAE / 14-3-3 Epsilon antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2948 concentration 5 ug/ml.

Anti-YWHAE / 14-3-3 Epsilon Antibody (Internal, clone EPR3919) - LS-C137795 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3919
Immunofluorescent staining of HeLa cells using anti-14-3-3E antibody.

Anti-YWHAE / 14-3-3 Epsilon Antibody (N-Terminus) IHC-plus™ - LS-B3683 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-YWHAE / 14-3-3 Epsilon antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3683 dilution 1:400.

Anti-YWHAE / 14-3-3 Epsilon Antibody (Asp238) - LS-C176846 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of 14-3-3 (D238) pAb in paraffin-embedded human brain tissue.

Anti-YWHAE / 14-3-3 Epsilon Antibody (aa1-255, clone 5A5) - LS-C63344 from LifeSpan Bioscience

Prices $355.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 5A5

Anti-YWHAE / 14-3-3 Epsilon Antibody (C-Terminus) - LS-C42371 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-YWHAE / 14-3-3 Epsilon Antibody (aa1-12) - LS-C121318 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-YWHAE / 14-3-3 Epsilon Antibody (aa239-255) - LS-C121319 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-YWHAE / 14-3-3 Epsilon Antibody (aa208-244) - LS-C126502 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-YWHAE / 14-3-3 Epsilon Antibody (aa208-244) - LS-C126503 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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