All lanes : Anti-14-3-3 sigma antibody (ab151504) at 1/3000 dilutionLane 1 : Neuro2A whole cell lysateLane 2 : GL261 whole cell lysateLysates/proteins at 30 µg per lane.
Anti-14-3-3 sigma antibody (ab151504) at 1/3000 dilution + PC12 whole cell lysate at 30 µg
All lanes : Anti-14-3-3 sigma antibody (ab151504) at 1/3000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : HeLa whole cell lysateLane 4 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.
Immunofluorescent analysis of A431 cells (fixed in ice-cold Methanol for 5 min), labeling 14-3-3 sigma with ab151504 at 1/500 dilution. The right image shows cells co-stained with Hoechst 33343.
Immunohistochemical analysis of paraffin-embedded Human 59T xenograft tissue, labeling 14-3-3 sigma with ab151504 at 1/100 dilution.