All lanes : Anti-14-3-3 sigma antibody [EPR5873(2)] (ab193667) at 1/1000 dilutionLane 1 : Human skin lysateLane 2 : A431 cell lysateLane 3 : HACAT cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-14-3-3 sigma antibody [EPR5873(2)] (ab193667) at 1/1000 dilution + Human 14-3-3 Sigma recombinant protein with His tag at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 14-3-3 sigma with ab193667 at 1/600 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.