All lanes : Anti-14-3-3 theta antibody (ab183075) at 1/300 dilutionLane 1 : mouse brain tissue lysateLane 2 : Human A549 cell lysateLane 3 : Human lymphoma tissue lysateLane 4 : mouse NIH 3T3 cell lysateLane 5 : Human colon cancer tissue lysateLysates/proteins at 40 µg per lane.SecondaryGoat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 14-3-3 theta with ab183075 at a 1/20 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)