YWHAQ

Gene Symbol YWHAQ
Entrez Gene 768311
Alt Symbol -
Species Bovine
Gene Type protein-coding
Description tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
Other Description 14-3-3 protein theta|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide|tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide
Swissprots A7E3X8 Q3SZI4
Accessions DAA24441 Q3SZI4 BC102840 AAI02841 BC151414 AAI51415 BT030749 ABS45065 NM_001078127 NP_001071595
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm {ECO:0000250}.
Top Pathways Cell cycle, Oocyte meiosis, Viral carcinogenesis, PI3K-Akt signaling pathway, Epstein-Barr virus infection

Anti-14-3-3 Tau antibody - ab77268 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-14-3-3 Tau antibody (ab77268) at 0.03 µg/ml + Human Brain (Hippocampus) in RIPA buffer at 35 µg

Anti-14-3-3 Tau antibody - ab52834 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-14-3-3 Tau antibody (ab52834) at 1/1000 dilutionLane 1 : a total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigenLane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein)Lysates/proteins at 20 µg per lane.SecondaryLane 1 : Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilutionLane 2 : Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution

Anti-14-3-3 Tau antibody - ab53200 from Abcam

Prices $359.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-14-3-3 Tau antibody [3B9] - ab10439 from Abcam

Prices $391.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3B9
14-3-3 Tau was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to 14-3-3 Tau and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10439.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.Band: 31kDa, non specific band - 26kDa: We are unsure as to the identity of this extra band; 14-3-3 Tau

Anti-14-3-3 theta antibody - ab183075 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-14-3-3 theta antibody (ab183075) at 1/300 dilutionLane 1 : mouse brain tissue lysateLane 2 : Human A549 cell lysateLane 3 : Human lymphoma tissue lysateLane 4 : mouse NIH 3T3 cell lysateLane 5 : Human colon cancer tissue lysateLysates/proteins at 40 µg per lane.SecondaryGoat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutiondeveloped using the ECL technique
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