Anti-5HT1A Receptor antibody
| Name | Anti-5HT1A Receptor antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab79230 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF WB IHC-P IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide from the 3d cytoplasmic domain of Mouse 5HT1A receptor, conjugated to an immunogenic carrier protein |
| Description | Rabbit Polyclonal |
| Gene | HTR1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-5HT1A Receptor antibody (ab79230) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab79230 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79230, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Neural circuit interactions between the dorsal raphe nucleus and the lateral - Neural circuit interactions between the dorsal raphe nucleus and the lateral
Jalewa J, Joshi A, McGinnity TM, Prasad G, Wong-Lin K, Holscher C. PLoS One. 2014 Feb 6;9(2):e88003.
Paracrine modulation of cholangiocyte serotonin synthesis orchestrates biliary - Paracrine modulation of cholangiocyte serotonin synthesis orchestrates biliary
Omenetti A, Yang L, Gainetdinov RR, Guy CD, Choi SS, Chen W, Caron MG, Diehl AM. Am J Physiol Gastrointest Liver Physiol. 2011 Feb;300(2):G303-15. doi: