Anti-5HT1B Receptor antibody
| Name | Anti-5HT1B Receptor antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab13896 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptides, corresponding to amino acids 8-26 (CAPPPPAGSETWVPQANLS) and 263-278 (STSSVTSINSRVPDVP) of 5HT1B Receptor |
| Description | Rabbit Polyclonal |
| Gene | HTR1B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab17976 staining 5HT1B in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBFand blocked with antibody block for 10 minutes; antigen retrieval was by heat mediation in CC1 Mild. Samples were incubated with primary antibody (1/100 in antibody diluent) for 1 hour at 37°C. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
ab2607 (4µg/ml) staining 5HT1B in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane compartment of the mature seminiferous tubules and possible interstitial cells.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
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