All lanes : Anti-5HT3B receptor antibody (ab39629) at 1/500 dilutionLane 1 : Lysate from HEK 293 cellsLane 2 : Lysate from HEK 293 cells transfected with 5HT3A receptorLane 3 : Lysate from HEK 293 cells transfected with 5HT3A receptor and 5HT3B receptorLane 4 : Human hippocampus lysate
HEK 293 cells transfected with 5HT3B receptor were stained with ab39629 diluted 1/500 and anti-rabbit secondary antibody conjugated to Alexa Fluor 488 diluted 1/1000(green). Nuclei are labelled with Hoescht 33258 (blue).
Immunohistochemistry of human hippocampus stained with ab39269 diluted 1/500. Immunoreactivity with 5HT3B receptor can be seen in (i)CA1 field, (ii)CA2, (iii)CA3 and (iv)CA4/dentate gyrus.