Anti-AATF antibody
| Name | Anti-AATF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab65248 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthetic peptide derived from N terminus of human AATF |
| Description | Rabbit Polyclonal |
| Gene | AATF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-AATF antibody (ab65248) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : HepG2 cell extractLane 3 : COLO205 cell extractLane 4 : HUVEC cell extractLane 5 : HUVEC cell extract with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.
ICC/IF image of ab65248 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65248, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.