Western blot analysis of MFRN2 Antibody (N-term) (Cat. #AP5244a) in Jurkat cell line lysates (35ug/lane).MFRN2 (arrow) was detected using the purified Pab.
MFRN2 Antibody (N-term) (Cat. #AP5244a) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MFRN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
MFRN2 Antibody (N-term) (Cat. #AP5244a) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-MFRN2 Antibody (N-term) at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.