Western blot analysis of lysates from HT-29, PC-3, U-87 MG cell line and human testis tissue lysate(from left to right), using OASL Antibody T499(Cat. #AP6230a). AP6230a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
The anti-OASL C-term Antibody (Cat.#AP6230a) is used in Western blot to detect OASL in HL60 lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.