Western blot analysis of anti-Gremlin Pab (Cat. #AP6133a) in CEM cell line lysates (35ug/lane). Gremlin (arrow) was detected using the purified Pab.
Western blot analysis of anti-Gremlin Pab (Cat. #AP6133a) in mouse liver cell lysate. Gremlin (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of primary antibody was 1:50. Detection method was ABC system or Vectastaint Elite VECTOR. Data and protocol courtesy of Dr. Maria E. Burgos of Universidad Austral de Chile.
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GREMLIN Antibody (C-term) (Cat.#AP6133a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
ISH and IMH demonstrating gremlin mRNA and gremlin protein in crescentic PECs and tubular epithelial cells of patients with pauci-immune crescentic GN. In comparison with normal renal tissue in which there is no expression of gremlin mRNA (C), proliferating PECs of glomerular crescents show a strong expression of gremlin mRNA by ISH (A); and gremlin protein expression by IMH (B); immune competent infiltrating interstitial cells are also strongly positive for gremlin staining (E); and CTGF was also expressed in these glomerular crescentic cells (F).