All lanes : Anti-Abhd5 antibody [EPR12621] (ab183739) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : U87-MG cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-Abhd5 antibody [EPR12621] (ab183739) at 1/1000 dilution + Human fetal liver lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human lipomyoma tissue labeling Abhd5 with ab183739 at 1/2000 dilution. The slide is counterstained with Hematoxylin.
Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling Abhd5 with ab183739 at 1/200 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.