Western blot analysis of VPS53 Antibody (N-term) (Cat. #AP8546a) in MCF-7 cell line lysates (35ug/lane). VPS53 (arrow) was detected using the purified Pab.
Western blot analysis of VPS53 Antibody (N-term) (Cat. #AP8546a) in mouse brain tissue lysates (35ug/lane). VPS53 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human brain tissue reacted with VPS53 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of MDA-MB468 cells using VPS53 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.