Western blot analysis of lysate from LNCap cell line, using CYP51A1 Antibody (Center)(Cat. #AP8874c). AP8874c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CYP51A1 Antibody (Center) (Cat. #AP8874c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
CYP51A1 Antibody (Center) (Cat. #AP8874c) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.