Western blot analysis of lysates from SW620, PC-3 cell line, mouse kidney, rat kidney tissue lysate (from left to right), using SUPV3L1 Antibody (N-term)(Cat. #AP9772a). AP9772a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Western blot analysis of SUPV3L1 Antibody (N-term) (Cat. #AP9772a) in K562 cell line lysates (35ug/lane). SUPV3L1 (arrow) was detected using the purified Pab.
SUPV3L1 Antibody (N-term) (Cat. #AP9772a) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SUPV3L1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
SUPV3L1 Antibody (N-term) (Cat. #AP9772a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.