ab74091 at 1/500 dilution staining ACK1 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
ab74091 at 1/50 dilution staining ACK1 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
All lanes : Anti-ACK1 (phospho Y284) antibody (ab74091) at 1/500 dilutionLane 1 : HepG2 cell extracts treated with EGF (200ng/ml, 30mins)Lane 2 : HepG2 cell extracts treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.