Anti-Aconitase 2 antibody

• Supplier: Abcam
• Catalog: ab71440
• Prices: $398.00
• Sizes: 400 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (Human) conjugated to KLH
• Description: Rabbit Polyclonal
• Gene: ACO2
• Conjugate: Unconjugated

Product images

All lanes : Anti-Aconitase 2 antibody (ab71440) at 1/60 dilutionLane 1 : Mouse heart tissue lysateLane 2 : 293 cell lysateLysates/proteins at 35 µg per lane.

All lanes : Anti-Aconitase 2 antibody (ab71440) at 1/1000 dilutionLane 1 : Perfused isolated rat heart whole tissue lysate lysed with 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail Lane 2 : Perfused isolated rat heart whole tissue lysate lysed with T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail Lysates/proteins at 12.5 µg per lane.

Ab71440 (2µg/ml) staining Aconitase 2 in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of Parietal cells.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

ICC/IF image of ab71440 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71440, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Mitochondria-targeted Ogg1 and aconitase-2 prevent oxidant-induced mitochondrial - Mitochondria-targeted Ogg1 and aconitase-2 prevent oxidant-induced mitochondrial

Kim SJ, Cheresh P, Williams D, Cheng Y, Ridge K, Schumacker PT, Weitzman S, Bohr VA, Kamp DW. J Biol Chem. 2014 Feb 28;289(9):6165-76.

Functional proteomic analysis reveals sex-dependent differences in structural and - Functional proteomic analysis reveals sex-dependent differences in structural and

Fogle RL, Hollenbeak CS, Stanley BA, Vary TC, Kimball SR, Lynch CJ. Physiol Genomics. 2011 Apr 12;43(7):346-56. doi:

Identification of a redox-modulatory interaction between uncoupling protein 3 and - Identification of a redox-modulatory interaction between uncoupling protein 3 and

Hirasaka K, Lago CU, Kenaston MA, Fathe K, Nowinski SM, Nikawa T, Mills EM. Antioxid Redox Signal. 2011 Nov 15;15(10):2645-61.