ACO2

Gene Symbol ACO2
Entrez Gene 50
Alt Symbol ACONM, ICRD, OCA8
Species Human
Gene Type protein-coding
Description aconitase 2, mitochondrial
Other Description aconitate hydratase, mitochondrial|citrate hydro-lyase
Swissprots Q6FHX0 O75809 Q8TAQ6 Q99798 Q5JZ41
Accessions AAC39921 AAD19351 EAW60435 EAW60436 Q99798 AA724140 AK293562 BAG57034 AK296260 BAG58973 AK297213 BAG59696 AK301321 BAG62870 AK302691 BAG63920 AK314845 BAG37362 BC014092 AAH14092 BC026196 AAH26196 CR456365 CAG30251 CR536568 CAG38805 CU012932 CAK54363 CU013220 CAK54662 DQ893674 ABM84600 DQ895836 ABM86762 U80040 AAB38416 NM_001098 NP_001089
Function Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. {ECO:0000250}.
Subcellular Location Mitochondrion {ECO:0000250}.
Top Pathways Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Biosynthesis of amino acids, 2-Oxocarboxylic acid metabolism, Carbon metabolism

ACO2 (D6D9) XP ® Rabbit mAb - 6571 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6D9
Western blot analysis of extracts from various cell lines using ACO2 (D6D9) XP ® Rabbit mAb.

ACO2 Antibody - 6922 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using ACO2 Antibody.

ACO2 (A-22) - sc-130677 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
ACO2 (A-22): sc-130677. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.

ACO2 (S-20) - sc-66426 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-Aconitase 2 antibody - ab71440 from Abcam

Prices $398.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Aconitase 2 antibody (ab71440) at 1/60 dilutionLane 1 : Mouse heart tissue lysateLane 2 : 293 cell lysateLysates/proteins at 35 µg per lane.

Anti-Aconitase 2 antibody - ab71439 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Aconitase 2 antibody (ab71439) at 1/1000 dilutionLane 1 : Rat heart whole tissue lysate lysed with 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail Lane 2 : Rat heart whole tissue lysate lysed with T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail Lysates/proteins at 12.5 µg per lane.

Anti-Aconitase 2 antibody - ab135806 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Aconitase 2 antibody (ab135806) at 1/50 dilutionLane 1 : 293 cell lysates, non-transfectedLane 2 : 293 cell lysates, transiently transfected with the Aconitase 2 gene Lysates/proteins at 2 µg per lane.

Anti-Aconitase 2 antibody - ab54719 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Aconitase 2 antibody [3G10] - ab118042 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3G10
ab118042 at 1/100 immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Aconitase 2.

Anti-Aconitase 2 antibody [6D1BE4] - ab110320 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6D1BE4
ab110320 at 1µg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Note: the target protein locates to the mitochondria.

Anti-Aconitase 2 antibody [6F12BD9] - ab110321 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6F12BD9
ab110321 at 1µg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).

Anti-Aconitase 2 antibody [6F12BD9] (Alexa Fluor® 488) - ab198049 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6F12BD9
ab198049 staining Aconitase 2 in WI38 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198049 at 1/500 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Aconitase 2 antibody [6F12BD9] (Alexa Fluor® 647) - ab198050 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6F12BD9
ab198050 staining Aconitase 2 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198050 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Aconitase 2 antibody [6F12BD9] (HRP) - ab198393 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6F12BD9
All lanes : Anti-Aconitase 2 antibody [6F12BD9] (HRP) (ab198393) at 1/5000 dilutionLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Aconitase 2 antibody [7G4] - ab175350 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7G4
All lanes : Anti-Aconitase 2 antibody [7G4] (ab175350) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A549 whole cell lysateLane 3 : Jurkat whole cell lysateLane 4 : A431 whole cell lysateLane 5 : MCF7 whole cell lysateLane 6 : HeLa whole cell lysateLane 7 : U205 whole cell lysateLane 8 : K562 whole cell lysateLane 9 : COS7 whole cell lysateLane 10 : 3T3L1 whole cell lysateLane 11 : NRK whole cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse-HRP at 1/20000 dilutiondeveloped using the ECL technique

Anti-Aconitase 2 antibody [EPR8282(B)] - ab129069 from Abcam

Prices $397.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8282(B)
All lanes : Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLane 3 : Human fetal heart lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-Aconitase 2 antibody [EPR8283(B)] - ab129105 from Abcam

Prices $397.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8283(B)
All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLane 3 : Jurkat cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-Aconitase 2 antibody [EPR8283(B)] (Alexa Fluor® 488) - ab197879 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8283(B)
Overlay histogram showing HeLa cells stained with ab197879 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197879, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Aconitase 2 antibody [EPR8283(B)] (Alexa Fluor® 647) - ab197880 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8283(B)
Overlay histogram showing HeLa cells stained with ab197880 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197880, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Aconitase 2 antibody [EPR8283(B)] (HRP) - ab198555 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8283(B)
All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (HRP) (ab198555) at 1/5000 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-ACO2 / Aconitase 2 Antibody (clone 7A11) - LS-C114914 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7A11
Anti-ACO2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2.

Anti-ACO2 / Aconitase 2 Antibody (aa541-555) IHC-plus™ - LS-B8320 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-ACO2 / Aconitase 2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8320 dilution 3.75 ug/ml.

Anti-ACO2 / Aconitase 2 Antibody (clone 7G4) - LS-C114938 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7G4
Immunofluorescent staining of HepG2 cells using anti-ACO2 mouse monoclonal antibody.

Anti-ACO2 / Aconitase 2 Antibody (aa554-566) IHC-plus™ - LS-B8319 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-ACO2 / Aconitase 2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8319 dilution 3.75 ug/ml.

Anti-ACO2 / Aconitase 2 Antibody (clone 3G8) - LS-C114898 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3G8
IHC of paraffin-embedded Ovary tissue using anti-ACO2 mouse monoclonal antibody. (Dilution 1:50).
Search more