Anti-Aconitase 2 antibody [6F12BD9]
| Name | Anti-Aconitase 2 antibody [6F12BD9] |
|---|---|
| Supplier | Abcam |
| Catalog | ab110321 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 6F12BD9 |
| Applications | IP ICC/IF ICC/IF IHC-F WB ELISA FC IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Bovine heart mitochondria |
| Description | Mouse Monoclonal |
| Gene | ACO2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab110321 at 1µg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
ab110321 at 1µg/ml staining Aconitase 2 in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).
![All lanes : Anti-Aconitase 2 antibody [6F12BD9] (ab110321) at 1 µg/mlLane 1 : HepG2 (human)Lane 2 : HeLa (human)Lane 3 : H9C2 (rat cardiomyocyte)Lane 4 : MEF (mouse fibroblast)Lysates/proteins at 20 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/1896_Aconitase-2-Primary-antibodies-ab110321-3.jpg)
All lanes : Anti-Aconitase 2 antibody [6F12BD9] (ab110321) at 1 µg/mlLane 1 : HepG2 (human)Lane 2 : HeLa (human)Lane 3 : H9C2 (rat cardiomyocyte)Lane 4 : MEF (mouse fibroblast)Lysates/proteins at 20 µg per lane.
IHC image of Aconitase 2 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110321, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
IHC-Fr image of Aconitase 2 staining on six month old rat muscle tissue using ab110321(1:1000).4% PFA fixed sections were incubated in 0.3% PBS-Tween for 1h. The sections were then incubated with ab110321 (1:1000) overnight at +4°C. The secondary antibody Alexa Fluor® 568 donkey anti-mouse was used at a 1/1000 dilution for 1h. DAPI was used at 1.43µMSee Abreview
Product References
Amino acid starvation has opposite effects on mitochondrial and cytosolic protein - Amino acid starvation has opposite effects on mitochondrial and cytosolic protein
Johnson MA, Vidoni S, Durigon R, Pearce SF, Rorbach J, He J, Brea-Calvo G, Minczuk M, Reyes A, Holt IJ, Spinazzola A. PLoS One. 2014 Apr 9;9(4):e93597.
High molecular weight forms of mammalian respiratory chain complex II. - High molecular weight forms of mammalian respiratory chain complex II.
Kovarova N, Mracek T, Nuskova H, Holzerova E, Vrbacky M, Pecina P, Hejzlarova K, Kluckova K, Rohlena J, Neuzil J, Houstek J. PLoS One. 2013 Aug 13;8(8):e71869.
Phosphoproteomic analysis of the striatum from pleiotrophin knockout and midkine - Phosphoproteomic analysis of the striatum from pleiotrophin knockout and midkine
Vicente-Rodriguez M, Gramage E, Herradon G, Perez-Garcia C. Toxicology. 2013 Dec 6;314(1):166-73.
Mutations in LYRM4, encoding iron-sulfur cluster biogenesis factor ISD11, cause - Mutations in LYRM4, encoding iron-sulfur cluster biogenesis factor ISD11, cause
Lim SC, Friemel M, Marum JE, Tucker EJ, Bruno DL, Riley LG, Christodoulou J, Kirk EP, Boneh A, DeGennaro CM, Springer M, Mootha VK, Rouault TA, Leimkuhler S, Thorburn DR, Compton AG. Hum Mol Genet. 2013 Nov 15;22(22):4460-73.