Anti-Aconitase 2 antibody [7G4]

• Supplier: Abcam
• Catalog: ab175350
• Prices: $384.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 7G4
• Applications: IHC-P WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human, Primate
• Antigen: Recombinant full length protein corresponding to Human Aconitase 2 aa 1-780
• Description: Mouse Monoclonal
• Gene: ACO2
• Conjugate: Unconjugated

Product images

All lanes : Anti-Aconitase 2 antibody [7G4] (ab175350) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A549 whole cell lysateLane 3 : Jurkat whole cell lysateLane 4 : A431 whole cell lysateLane 5 : MCF7 whole cell lysateLane 6 : HeLa whole cell lysateLane 7 : U205 whole cell lysateLane 8 : K562 whole cell lysateLane 9 : COS7 whole cell lysateLane 10 : 3T3L1 whole cell lysateLane 11 : NRK whole cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse-HRP at 1/20000 dilutiondeveloped using the ECL technique

Immunohistochemical analysis of cancer biopsies of deparaffinized Human colon, myocardial, hepatic and renal cell carcinoma tissues labeling Aconitase 2 with ab175350 at 1/800 dilution. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin and prepped for mounting.

Immunofluorescent analysis of formalin-fixed HeLa cells labeling Aconitase 2 with ab175350 at 1/100 dilution (green). Detection utilised DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1/400. Nuclei (blue) were stained with Hoechst 33342 dye.

All lanes : Anti-Aconitase 2 antibody [7G4] (ab175350) at 1/1000 dilutionLane 1 : Whole cell lysate from control pool siRNA transfected MCF7 cellsLane 2 : Whole cell lysate from Aconitase 2 SMART pool siRNA transfected MCF7 cellsLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse-HRP at 1/20000 dilutiondeveloped using the ECL technique