All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLane 3 : Jurkat cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution
ab129105, at 1/100 dilution, staining Aconitase 2 in Paraffin-embedded Human kidney tissue by immunohistochemistry.
All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/1000 dilutionLane 1 : 1 month old Liver Mitochondrial fractionLane 2 : 3 month old Liver Mitochondrial fractionLane 3 : 3 month old Liver Mitochondrial fractionLane 4 : 6 month old Liver Mitochondrial fractionSecondaryPig Polyclonal to anti-Rabbit (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC-Fr image of Aconitase 2 staining of six month old rat skeletal muscles using ab129105 (1:500). 4% PFA fixed sections were incubated in 0.3% PBS-Tween for 1h. The sections were then incubated with the antibody (ab129105) overnight at +4°C. The secondary antibody Alexa Fluor® 488 donkey anti-rabbit (1:1000) for 1h. DAPI was used at a concentration of 1.43µM. See Abreview
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD