All lanes : Anti-ACSL1 antibody (ab89854) at 1 µg/mlLane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 4 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.