Anti-active + pro Caspase 3 antibody
| Name | Anti-active + pro Caspase 3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab47131 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB IHC-F IP |
| Species Reactivities | Mouse, Rat, Human, Drosophila, Rat |
| Antigen | Recombinant full length protein corresponding to Human active + pro Caspase 3 |
| Description | Rabbit Polyclonal |
| Gene | CASP3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-active + pro Caspase 3 antibody (ab47131) at 1/1000 dilutionLane 1 : Lysates from Jurkat cells, total protein content (50 ug/lane)Lane 2 : Normal mammary tissue, total protein content (50 ug/lane)Lane 3 : Surgical specimens from one invasive ductal carcinomas, total protein content (50 ug/lane)Lane 4 : Surgical specimens from one invasive ductal carcinomas, total protein content (50 ug/lane)Lane 5 : Surgical specimens from one invasive ductal carcinomas, total protein content (50 ug/lane)
Immunohistochemical analysis of Caspase 3 expression in formalin fixed, paraffin embedded human reactive lymph node using ab47131 at 1/2000. Staining is seen in the apoptosis prone germinal center B lymphocytes of follicles. In constrast little or no staining is seen in the surrounding long live mantle zone lymphocytes.
Immunohistochemical analysis of Caspase 3 expression in formalin fixed, paraffin embedded human breast ductal carcinoma in situ using ab47131 at 1/2000. Staining is seen in the the cancerous ducts, but not in the normal lobulus.
ICC/IF image of ab47131 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47131, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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