Fluorescent image of SH-SY5Y cells stained with ANP32E Antibody (N-term)(Cat#AP20559a). AP20559a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from SH-SY5Y, U-937 cell line and mouse brain tissue lysate (from left to right), using ANP32E Antibody (N-term) (Cat. #AP20559a). AP20559a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.