Immunohistochemical analysis of paraffin-embedded H. kidney section using MAD2L2 Antibody (C-term)(Cat#AP20654c). AP20654c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Fluorescent image of Hela cells stained with MAD2L2 Antibody (C-term)(Cat#AP20654c). AP20654c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from Hela, K562 cell line (from left to right), using MAD2L2 Antibody (C-term)(Cat. #AP20654c). AP20654c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.