Anti-ADAM10 antibody [EPR5622]
| Name | Anti-ADAM10 antibody [EPR5622] |
|---|---|
| Supplier | Abcam |
| Catalog | ab124695 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR5622 |
| Applications | WB IP FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide, corresponding to residues from the C-terminus of Human ADAM10 |
| Description | Rabbit Monoclonal |
| Gene | ADAM10 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing HeLa cells stained with ab124695 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124695, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : RAW 264.7 cell lysateLane 3 : LnCaP cell lysateLane 4 : MCF-7 cell lysateLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/2547_ADAM10-Primary-antibodies-ab124695-1.jpg)
All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : RAW 264.7 cell lysateLane 3 : LnCaP cell lysateLane 4 : MCF-7 cell lysateLysates/proteins at 10 µg per lane.
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD
Product References
Enhancement of the nonamyloidogenic pathway by exogenous NGF in an Alzheimer - Enhancement of the nonamyloidogenic pathway by exogenous NGF in an Alzheimer
Yang C, Liu Y, Ni X, Li N, Zhang B, Fang X. Neuropeptides. 2014 Aug;48(4):233-8.
Adrenergic regulation of IgE involves modulation of CD23 and ADAM10 expression on - Adrenergic regulation of IgE involves modulation of CD23 and ADAM10 expression on
Padro CJ, Shawler TM, Gormley MG, Sanders VM. J Immunol. 2013 Dec 1;191(11):5383-97.