ADAM10

Gene Symbol ADAM10
Entrez Gene 102
Alt Symbol AD10, AD18, CD156c, HsT18717, MADM, RAK, kuz
Species Human
Gene Type protein-coding
Description ADAM metallopeptidase domain 10
Other Description CDw156|a disintegrin and metalloprotease domain 10|disintegrin and metalloproteinase domain-containing protein 10|kuzbanian protein homolog|mammalian disintegrin-metalloprotease
Swissprots Q10742 B4DU28 Q92650 O14672
Accessions CCQ43088 EAW77539 EAW77540 EAW77541 O14672 AA243020 AF009615 AAC51766 AK023460 AK300472 BAG62190 AY726599 BC055087 BC104659 BC126253 AAI26254 BC143935 BE327886 BE934813 BX372217 DA941750 Z48579 CAA88463 XM_005254117 XP_005254174 NM_001110 NP_001101
Function Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3
Subcellular Location Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein. Note=Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
Tissue Specificity Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver. {ECO:0000269|PubMed:11511685, ECO:0000269|PubMed:9016778}.
Top Pathways Alzheimer's disease, Epithelial cell signaling in Helicobacter pylori infection

ADAM10 Antibody - 14194 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using ADAM10 Antibody.

ADAM10 (A-3) - sc-48400 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ADAM10 (A-3): sc-48400. Western blot analysis of ADAM10 expression in HuT 78 (A), Jurkat (B) and NIH/3T3 (C) whole cell lysates.

ADAM10 (B-3) - sc-28358 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ADAM10 (B-3): sc-28358. Western blot analysis of ADAM10 expression in MH-S (A), Jurkat (B), PC-12 (C) and KNRK (D) whole cell lysates.

ADAM10 (H-300) - sc-25578 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ADAM10 (H-300): sc-25578. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.

ADAM10 (A-20) - sc-16524 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ADAM10 (A-20): sc-16524. Western blot analysis of ADAM10 expression in MH-S (A), 3T3-L1 (B), NIH/3T3 (C) and U-937 (D) whole cell lysates.

ADAM10 (T-17) - sc-31855 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ADAM10 (T-17): sc-31855. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cartilage tissue showing cytoplasmic and membrane staining of chondrocytes.

ADAM10 (S-20) - sc-16523 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ADAM10 (S-20): sc-16523. Immunofluorescence staining of methanol-fixed MH-S cells showing cytoplasmic and nuclear localization.

ADAM10 (V-20) - sc-31853 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ADAM10 (V-20): sc-31853. Western blot analysis of ADAM10 expression in MH-S (A), 3T3-L1 (B), NIH/3T3 (C) and U-937 (D) whole cell lysates.

Anti-ADAM10 antibody - ab1997 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab1997 at 2µg/ml staining K562 cells by ICC/IF.

Anti-ADAM10 antibody - ab39178 from Abcam

Prices $387.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Methanol fixed Hela cells stained with ab39178 (10 µg/ml) for 1 hour at 22°C. For further details please refer to the Abreview.

Anti-ADAM10 antibody - ab39180 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Methanol fixed HeLa cells were incubated with ab39180 (10 µg/ml) for 1 hour at 22°C. For further details please refer to the Abreview.

Anti-ADAM10 antibody [11G2] - ab59482 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 11G2
IHC image of ab59482 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59482, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ADAM10 antibody [EPR5622] - ab124695 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5622
Overlay histogram showing HeLa cells stained with ab124695 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124695, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-ADAM10 antibody [MM0077-6D31] - ab89122 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MM0077-6D31

Anti-ADAM10 antibody - Aminoterminal end - ab39153 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab39153 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39153, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ADAM10 Antibody - LS-C48244 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ADAM10 Antibody (aa214-672) - LS-C122598 from LifeSpan Bioscience

Prices $605.00
Sizes 1 mg
Host Goat
Clonality Polyclonal

Anti-ADAM10 Antibody - LS-C124857 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-ADAM10 Antibody (CF) - LS-C124902 from LifeSpan Bioscience

Prices $445.00
Sizes 100 tst
Host Mouse
Clonality Monoclonal

Anti-ADAM10 Antibody (PE) - LS-C124903 from LifeSpan Bioscience

Prices $530.00
Sizes 100 tst
Host Mouse
Clonality Monoclonal

Anti-ADAM10 Antibody (clone 163003, APC) - LS-C124901 from LifeSpan Bioscience

Prices $590.00
Sizes 100 tst
Host Mouse
Clonality Monoclonal
Clone 163003

Anti-ADAM10 Antibody (clone 2009C3a) - LS-C184611 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2009C3a

Anti-ADAM10 Antibody (aa732-748) - LS-C188850 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-ADAM10 Antibody (aa420-641, Biotin) - LS-C300616 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ADAM10 Antibody (aa218-445, Biotin) - LS-C300618 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Search more