Western blot analysis of lysate from A549 cell line, using CHSY3 Antibody (Center)(Cat. #AP21080a). AP21080a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
All lanes : Anti-CHSY3 Antibody (Center) at 1:1000-1:2000 dilution Lane 1: mouse brain lysates Lane 2: human brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 100 kDa Blocking/Dilution buffer: 5% NFDM/TBST.