Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate: ab173579 at 1/10 immunoprecipitated ADAM17.
Flow cytometric analysis of permeabilized Jurkat cells labeling ADAM17 with ab173579 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
Immunofluorescent staining of HeLa cells labeling ADAM17 with ab173579 at 1/50 dilution
All lanes : Anti-ADAM17 antibody [EPR3600(N)(B)] (ab173579) at 1/1000 dilutionLane 1 : Raji cell lysateLane 2 : Jurkat cell lysateLane 3 : Hela cell lysateLysates/proteins at 10 µg per lane.