ADAM17

Gene Symbol ADAM17
Entrez Gene 6868
Alt Symbol ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE
Species Human
Gene Type protein-coding
Description ADAM metallopeptidase domain 17
Other Description ADAM metallopeptidase domain 18|TNF-alpha convertase|TNF-alpha converting enzyme|disintegrin and metalloproteinase domain-containing protein 17|snake venom-like protease|tumor necrosis factor, alpha, converting enzyme
Swissprots P78536 O60226
Accessions AAX93091 AAX93142 AAY14859 EAX00981 EAX00982 P78536 AK289829 BAF82518 AY422721 BC062687 AAH62687 BC136783 AAI36784 BC146658 AAI46659 BM725368 BQ186514 BX339672 CN258241 GQ402545 ACV30013 U69611 AAB51514 U69612 AAB53014 U86755 AAB51586 U92649 AAC39721 XM_011510375 XP_011508677 XM_011510376 XP_011508678 XM_011510377 XP_011508679 XM_011510378 XP_011508680 NM_003183 NP_003174
Function Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. {ECO:0000269|PubMed:12441351, ECO:0000269|PubMed:20592283, ECO:0000269|PubMed:24226769, ECO:0000269|PubMed:24227843}.
Subcellular Location Membrane; Single-pass type I membrane protein.
Tissue Specificity Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
Top Pathways Notch signaling pathway, Alzheimer's disease, Epithelial cell signaling in Helicobacter pylori infection

TACE (D22H4) Rabbit mAb - 6978 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D22H4
Western blot analysis of extracts from Raji and Jurkat cells, untreated or treated with peptide N-glycosidase F (PNGase F), using TACE (D22H4) Rabbit mAb.

TACE Antibody - 3976 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines using TACE Antibody.

TACE (B-6) - sc-390859 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TACE (B-6): sc-390859. Western blot analysis of TACE expression in NAMALWA (A), K-562 (B), MOLT-4 (C), BJAB (D) and CCRF-HSB-2 (E) whole cell lysates.

TACE (K-20) - sc-6417 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TACE (K-20): sc-6417. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells.

TACE (C-15) - sc-6416 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TACE (C-15): sc-6416. Western blot analysis of TACE expression in BJAB (A), NAMALWA (B), MOLT-4 (C), CCRF-HSB-2 (D) and K-562 (E) whole cell lysates.

TACE (H-300) - sc-13973 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TACE (H-300): sc-13973. Western blot analysis of TACE expression in NAMALWA (A) and K-562 (B) whole cell lysates.

TACE (H-170) - sc-25782 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TACE (H-170): sc-25782. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic and membrane staining of glandular cells.

Anti-ADAM17 (phospho T735) antibody - ab182630 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ADAM17 (phospho T735) antibody (ab182630) at 1/500 dilutionLane 1 : Extracts from K562 cells treated with UV with Antigen specific peptideLane 2 : Extracts from K562 cells treated with UVdeveloped using the ECL technique

Anti-ADAM17 (phospho T735) antibody - ab195828 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ADAM17 (phospho T735) antibody (ab195828) at 1/500 dilutionLane 1 : UV treated K562 whole cell lysate with antigen-specific peptideLane 2 : UV treated K562 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-ADAM17 antibody - ab2051 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ADAM17 antibody (ab2051) at 1/500 dilutionLane 1 : HeLa whole cell lysate with absence of blocking peptideLane 2 : Jurkat whole cell lysate with absence of blocking peptideLane 3 : HeLa whole cell lysate with ADAM17 peptide (ab7881)Lane 4 : Jurkat whole cell lysate with ADAM17 peptide (ab7881)

Anti-ADAM17 antibody - ab57484 from Abcam

Prices $398.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ADAM17 antibody (ab57484) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.

Anti-ADAM17 antibody - ab28233 from Abcam

Prices $388.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Methanol fixed Hela cells stained with ab28233 (10 µg/ml) for 1 hour at 22°C. Clear staining can be seen. For further details please refer to the Abreview

Anti-ADAM17 antibody - ab13535 from Abcam

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-ADAM17 antibody (ab13535) at 0.2 µg/ml + Hela lysate (RIPA buffer, 35µg total protein per lane)

Anti-ADAM17 antibody - ab75609 from Abcam

Prices $378.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal
Anti-ADAM17 antibody (ab75609) at 5 µg/ml + HeLa cell lysate

Anti-ADAM17 antibody - ab39161 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Methanol fixed HeLa cells incubated with ab39161 at 10 µg/ml for 1 hour at 22°C. Clear membrane staining can be seen. For further details please refer to the Abreview.

Anti-ADAM17 antibody - ab78162 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab78162, used in immunohistochemistry at 20 &microg/ml on formalin-fixed, paraffin embedded skeletal muscle

Anti-ADAM17 antibody - ab75181 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ADAM17 antibody (ab75181) at 1/500 dilutionLane 1 : extracts from rat liver cellsLane 2 : extracts from rat liver cells with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-ADAM17 antibody - ab11547 from Abcam

Prices $359.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-ADAM17 antibody - ab151770 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ADAM17 antibody [1F6] - ab119290 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1F6
ab119290, at 5 µg/ml, staining ADAM17 in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry.

Anti-ADAM17 antibody [EPR3600(N)(B)] - ab173579 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3600(N)(B)
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate:  ab173579 at 1/10 immunoprecipitated ADAM17.

Anti-ADAM17 antibody [EPR3600(N)(B)] (Alexa Fluor® 488) - ab196457 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3600(N)(B)
ab196457 staining ADAM17 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab196457 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 100% methanol (5 min) fixed SKNSH cells under the same testing conditions.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-ADAM17 antibody [EPR3600(N)(B)] (Alexa Fluor® 647) - ab196581 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3600(N)(B)
ab196581 staining ADAM17 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196581 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Anti-ADAM17 antibody - Activation site - ab39163 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
The image shows ab39163 detecting mouse ADAM17 expressed in transfected HeLa cells. The cells were fixed in methanol and incubated with 10 µg/ml ab39163 for 1 hour at 20°C.

Anti-ADAM17 antibody - Cytoplasmic domain - ab39162 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab39162 was used to detect mouse ADAM17 transiently overexpressed in Hela cells. Ab39162 was incubated with the cells at 10 µg/ml for 1 hour at 22°C. For further details please refer to the Abreview.
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