Anti-ADAM17 antibody - Activation site
| Name | Anti-ADAM17 antibody - Activation site |
|---|---|
| Supplier | Abcam |
| Catalog | ab39163 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Pig |
| Antigen | Synthetic peptide based on the activation site (cysteine switch and furin cleavage site) of human ADAM17 |
| Description | Rabbit Polyclonal |
| Gene | ADAM17 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
The image shows ab39163 detecting mouse ADAM17 expressed in transfected HeLa cells. The cells were fixed in methanol and incubated with 10 µg/ml ab39163 for 1 hour at 20°C.
Anti-ADAM17 antibody - Activation site (ab39163) at 1/4000 dilution + MDA-MB231 lysateSecondaryDonkey anti-Rabbit at 1/10000 dilution
Ab39163 staining human normal colon tissue. Staining is localised to cellular membranes.Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Product References
Vascular induction of a disintegrin and metalloprotease 17 by angiotensin II - Vascular induction of a disintegrin and metalloprotease 17 by angiotensin II
Obama T, Takayanagi T, Kobayashi T, Bourne AM, Elliott KJ, Charbonneau M, Dubois CM, Eguchi S. Am J Hypertens. 2015 Jan;28(1):10-4.
A role for cGMP in inducible nitric-oxide synthase (iNOS)-induced tumor necrosis - A role for cGMP in inducible nitric-oxide synthase (iNOS)-induced tumor necrosis
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A disintegrin and metalloprotease 17 mediates neointimal hyperplasia in - A disintegrin and metalloprotease 17 mediates neointimal hyperplasia in
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Synaptic autoregulation by metalloproteases and gamma-secretase. - Synaptic autoregulation by metalloproteases and gamma-secretase.
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Arecoline-induced death of human leukemia K562 cells is associated with surface - Arecoline-induced death of human leukemia K562 cells is associated with surface
Chen YJ, Chang LS. J Cell Physiol. 2012 May;227(5):2240-51.
Suppression of ADAM17-mediated Lyn/Akt pathways induces apoptosis of human - Suppression of ADAM17-mediated Lyn/Akt pathways induces apoptosis of human
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Taiwan cobra phospholipase A2 elicits posttranscriptional up-regulation of ADAM17 - Taiwan cobra phospholipase A2 elicits posttranscriptional up-regulation of ADAM17
Liu WH, Chen KC, Chiou YL, Lin SR, Chang LS. J Cell Biochem. 2010 Sep 1;111(1):148-57.
ADAM17 co-purifies with TIMP-3 and modulates endothelial invasion responses in - ADAM17 co-purifies with TIMP-3 and modulates endothelial invasion responses in
Kwak HI, Mendoza EA, Bayless KJ. Matrix Biol. 2009 Oct;28(8):470-9.