Western blot analysis of rat brain lysates: 1. Anti-NMDA Receptor 2B (GluN2B) (extracellular) antibody (#AG1265), (1:600). 2. Anti-NMDA Receptor 2B (GluN2B) (extracellular) antibody, preincubated with the control peptide antigen.
Immunoprecipitation of rat brain lysates:
Expression of NMDA Receptor 2B in rat hippocampus Immunohistochemical staining of rat hippocampal CA1 frozen sections stained with Anti-NMDA Receptor 2B (GluN2B) (extracellular) antibody (#AG1265), (1:100). A. NMDA Receptor 2B (green) appears in the pyramidal layer of CA1. B. Staining of neurofilament 200 (red) identifies neuronal processes. C. Confocal merge demonstrates localization of NMDA Receptor 2B in cells and not in processes.
Expression of NMDA Receptor 2B in rat cortex Immunohistochemical staining of rat parietal cortex frozen sections stained with Anti-NMDA Receptor 2B (GluN2B) (extracellular) antibody (#AG1265), (1:100). A. NMDA Receptor 2B (green) appears in the pyramidal layer of layer 5. B. Staining of neurofilament 200 (red) identifies neuronal processes. C. Confocal merge demonstrates localization of NMDA Receptor 2B in cells.