Anti-ADAMTS13 antibody
| Name | Anti-ADAMTS13 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28273 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide based on the carboxyterminal end of full length human ADAMTS13 |
| Description | Rabbit Polyclonal |
| Gene | ADAMTS13 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-ADAMTS13 antibody (ab28273) at 1 µg/mlLane 1 : Recombinant Human ATS-13 at 0.08 µgLane 2 : Recombinant Human ATS-13 at 0.04 µgLane 3 : Recombinant Human ATS-13 at 0.02 µg
ICC/IF image of ab28273 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28273, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.