Anti-ADAMTS5 antibody
| Name | Anti-ADAMTS5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab41037 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human ADAMTS5 |
| Description | Rabbit Polyclonal |
| Gene | ADAMTS5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab41037 stained MG-63 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41037 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-ADAMTS5 antibody (ab41037) at 1/250 dilutionLane 1 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lane 2 : Placenta (Mouse) Whole Cell Lysate - normal tissue (ab29139)Lane 3 : Uterus (Human) Whole Cell Lysate - normal tissueLane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
All lanes : Anti-ADAMTS5 antibody (ab41037) at 1/250 dilutionLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 3 : Brain (Mouse) Tissue LysateLane 4 : Liver (Mouse) Tissue Lysate - normal tissueLane 5 : Testis (Mouse) Tissue Lysate - normal tissueLane 6 : Spinal Cord (Mouse) Tissue LysateLane 7 : Ovary (Mouse) Tissue Lysate - normal tissueLane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 9 : Brain (Rat) Tissue Lysate - normal tissueLane 10 : Liver (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
All lanes : Anti-ADAMTS5 antibody (ab41037) at 1 µg/ml (blocked with 3% Milk)Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab41037 staining ADAMTS5 in mouse knee joint tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and an enzymatic mediated antigen retrieval step was performed using Proteinase K. Samples were then blocked with 5% serum for 10 minutes at room temperature followed by incubation with the primary antibody at 10µg/ml for 1 hour. An undiluted HRP-conjugated human anti-rabbit monoclonal was used as secondary antibody.See Abreview
Product References
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