Anti-ADAMTS5 antibody - Catalytic domain
| Name | Anti-ADAMTS5 antibody - Catalytic domain |
|---|---|
| Supplier | Abcam |
| Catalog | ab50749 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Human |
| Antigen | The antibody is made to a synthetic peptide corresponding to aa 280-350 of the aminoterminal end (catalytic domain) of human ADAMTS-5 |
| Description | Rabbit Polyclonal |
| Gene | ADAMTS5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-ADAMTS5 antibody - Catalytic domain (ab50749) at 1/1000 dilutionLane 1 : rH-ADAMTS-5 Catalytic Domain at 0.1 µgLane 2 : rH-ADAMTS-5 Catalytic Domain at 0.04 µgLane 3 : rH-ADAMTS-5 Catalytic Domain at 0.02 µg
IHC image of ab50749 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50749, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.