All lanes : Anti-ADAR1 antibody (ab168809) at 0.4 µg/mlLane 1 : 293T whole cell lysate at 50 µgLane 2 : 293T whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique
Detection of ADAR1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168809 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling ADAR1 with ab168809 at 1/1000 (1µg/ml). Detection: DAB.