![All lanes : Anti-ADAR1 antibody [EPR7033] (ab126745) at 1/1000 dilutionLane 1 : HeLa (treated with IFN-alpha) cell lysateLane 2 : HeLa cell lysateLane 3 : Ramos cell lysateLane 4 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/2824_ADAR1-Primary-antibodies-ab126745-1.jpg)
All lanes : Anti-ADAR1 antibody [EPR7033] (ab126745) at 1/1000 dilutionLane 1 : HeLa (treated with IFN-alpha) cell lysateLane 2 : HeLa cell lysateLane 3 : Ramos cell lysateLane 4 : SH-SY5Y cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
ab126745, at 1/50 dilution, staining ADAR1 in paraffin-embedded Human brain tissue by Immunohistochemistry.
ab126745, at 1/50 dilution, staining ADAR1 in Hela cells by Immunofluorescence.
Flow cytometric analysis of permeabilized Ramos cells, staining ADAR1 (red) with ab126745. 1x106 cells were collected and washed with blocking buffer. Cells were fixed with 2% paraformaldehyde, permeabilized with 1X FACS permeabilizing solution and blocked with blocking buffer for 30 minutes at room temperature. Cells were incubated with primary antibody (1/10) for 30 minutes at room temperature before a fluorescently-conjugated secondary antibody or 30 min at room temperature. A rabbit IgG was used as a negative control (green).