Anti-Adiponectin antibody [19F1]
| Name | Anti-Adiponectin antibody [19F1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab22554 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | 19F1 |
| Applications | ELISA ICC/IF ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Rat, Rabbit, Human, Monkey |
| Antigen | Recombinant full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | ADIPOQ |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab22554 staining Adiponectin in 3T3-L1 cells (ATCC® CL-173 TM). Increased expression of Adiponectin correlates with adipocyte phenotype, as described in literature. Cells, grown to confluency in DMEM with 10% FBS, were differentiated by stimulation for two days with 0.5 mM 3-isobutyl-1-methylxanthine (ab120840), 0.25uM dexamethasone (ab120743) and 1ug/ml insulin (ab123768), followed by two more days with only insulin. Cells were maintained for an additional three days in growth medium alone. Undifferentiated and differentiated adipocytes were fixed with 100% methanol (5min) at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature. Staining of the treated cells with ab22554 (2.5µg/ml) and ab6046 at 1µg/ml overnight at +4°C, was followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 2 µg/ml (shown in green) and AlexaFluor®594 Goat anti-Mouse sec
![Anti-Adiponectin antibody [19F1] (ab22554) at 1 µg/ml + 3T3-L1 (Mouse embryonic fibroblast - adipose like cell line) Nuclear Lysate (ab14632)](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/3194_ab22554.jpg)
Anti-Adiponectin antibody [19F1] (ab22554) at 1 µg/ml + 3T3-L1 (Mouse embryonic fibroblast - adipose like cell line) Nuclear Lysate (ab14632)
ab22554 staining Adiponectin in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/200) was used as the secondary antibody.See Abreview
ab22554 (4µg/ml) staining adiponectin in human breast, using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear and cytoplasmic staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ab22554 staining Adiponectin in human adipose stem cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X and then blocked using 4% serum for 1 hour. Samples were then incubated with primary antibody at 1/500 for 1 hour 30 minutes. The secondary antibody used was a goat IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.See Abreview
Immunohistochemistry was performed on biopsies of deparaffinized Human skin tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Adiponectin ab22554 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Adiponectin ab22554 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Product References
Identification of a distinct small cell population from human bone marrow reveals - Identification of a distinct small cell population from human bone marrow reveals
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Adiponectin is sufficient, but not required, for exercise-induced increases in - Adiponectin is sufficient, but not required, for exercise-induced increases in
Ritchie IR, MacDonald TL, Wright DC, Dyck DJ. J Physiol. 2014 Jun 15;592(Pt 12):2653-65.
Adiponectin is not required for exercise training-induced improvements in glucose - Adiponectin is not required for exercise training-induced improvements in glucose
Ritchie IR, Wright DC, Dyck DJ. Physiol Rep. 2014 Sep 11;2(9). pii: e12146.
cAMP-responsive element binding protein: a vital link in embryonic hormonal - cAMP-responsive element binding protein: a vital link in embryonic hormonal
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Shotgun proteomics identifies serum fibronectin as a candidate diagnostic - Shotgun proteomics identifies serum fibronectin as a candidate diagnostic
Brown JK, Lauer KB, Ironmonger EL, Inglis NF, Bourne TH, Critchley HO, Horne AW. PLoS One. 2013 Jun 24;8(6):e66974.
Kruppel-like factor KLF9 regulates PPARgamma transactivation at the middle stage - Kruppel-like factor KLF9 regulates PPARgamma transactivation at the middle stage
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Interleukin-1beta may mediate insulin resistance in liver-derived cells in - Interleukin-1beta may mediate insulin resistance in liver-derived cells in
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Docosahexaenoic acid increases cellular adiponectin mRNA and secreted adiponectin - Docosahexaenoic acid increases cellular adiponectin mRNA and secreted adiponectin
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Adiponectin resistance precedes the accumulation of skeletal muscle lipids and - Adiponectin resistance precedes the accumulation of skeletal muscle lipids and
Mullen KL, Pritchard J, Ritchie I, Snook LA, Chabowski A, Bonen A, Wright D, Dyck DJ. Am J Physiol Regul Integr Comp Physiol. 2009 Feb;296(2):R243-51. doi:
Human CD34/CD90 ASCs are capable of growing as sphere clusters, producing high - Human CD34/CD90 ASCs are capable of growing as sphere clusters, producing high
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