AF3160a (10 µg/ml) staining of Human renal cortical brush-border membrane lysate (90 µg protein denatured at 37C for 30min) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was overnight at 4C. Detected by NBT/BCIP. Data kindly provided by Dean Karaica, B. Sc., and Ivan Sabolic, M.D., Ph.D., Institute for Medical Research and Occupational Health, Zagreb, Croatia.
AF3160a (0.1mg/ml) overnight staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, CY3-staining. Data kindly provided by Dean Karaica, B. Sc., and Ivan Sabolic, M.D., Ph.D., Institute for Medical Research and Occupational Health, Zagreb, Croatia.