Anti-Adipose Triglyceride Lipase antibody [AT2G2]
| Name | Anti-Adipose Triglyceride Lipase antibody [AT2G2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab85348 |
| Prices | $376.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | AT2G2 |
| Applications | FC WB ELISA |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment, corresponding to amino acids 30-504 (Lumenal domain) of Human Adipose Triglyceride Lipase purified from E |
| Description | Mouse Monoclonal |
| Gene | PNPLA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Adipose Triglyceride Lipase antibody [AT2G2] (ab85348) at 1/1000 dilution + mouse kidney extract at 20 µgSecondary goat anti-mouse HRPdeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/3266_Adipose-Triglyceride-Lipase-Primary-antibodies-ab85348-1.jpg)
Anti-Adipose Triglyceride Lipase antibody [AT2G2] (ab85348) at 1/1000 dilution + mouse kidney extract at 20 µgSecondary goat anti-mouse HRPdeveloped using the ECL technique
![Overlay histogram showing A431 cells stained with ab85348 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85348, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/3267_Adipose-Triglyceride-Lipase-Primary-antibodies-ab85348-2.jpg)
Overlay histogram showing A431 cells stained with ab85348 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85348, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.