Anti-AFF4 antibody
| Name | Anti-AFF4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57077 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | IHC-P WB FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 1-109 of Human AFF4 |
| Description | Mouse Monoclonal |
| Gene | AFF4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
AFF4 antibody (ab57077) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human endometrium.
Western blot against tagged recombinant protein immunogen using ab57077 AFF4 antibody at 1ug/ml.
![Overlay histogram showing HeLa cells stained with ab57077 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57077, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/3472_AFF4-Primary-antibodies-ab57077-1.jpg)
Overlay histogram showing HeLa cells stained with ab57077 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57077, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
AFF1 is a ubiquitous P-TEFb partner to enable Tat extraction of P-TEFb from 7SK - AFF1 is a ubiquitous P-TEFb partner to enable Tat extraction of P-TEFb from 7SK
Lu H, Li Z, Xue Y, Schulze-Gahmen U, Johnson JR, Krogan NJ, Alber T, Zhou Q. Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):E15-24.
Release of positive transcription elongation factor b (P-TEFb) from 7SK small - Release of positive transcription elongation factor b (P-TEFb) from 7SK small
Liu P, Xiang Y, Fujinaga K, Bartholomeeusen K, Nilson KA, Price DH, Peterlin BM. J Biol Chem. 2014 Apr 4;289(14):9918-25.
The elongation complex components BRD4 and MLLT3/AF9 are transcriptional - The elongation complex components BRD4 and MLLT3/AF9 are transcriptional
Flajollet S, Rachez C, Ploton M, Schulz C, Gallais R, Metivier R, Pawlak M, Leray A, Issulahi AA, Heliot L, Staels B, Salbert G, Lefebvre P. PLoS One. 2013 Jun 10;8(6):e64880.
Bromodomain and extra-terminal (BET) bromodomain inhibition activate - Bromodomain and extra-terminal (BET) bromodomain inhibition activate
Bartholomeeusen K, Xiang Y, Fujinaga K, Peterlin BM. J Biol Chem. 2012 Oct 19;287(43):36609-16.
HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a - HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a
He N, Liu M, Hsu J, Xue Y, Chou S, Burlingame A, Krogan NJ, Alber T, Zhou Q. Mol Cell. 2010 May 14;38(3):428-38.