All lanes : Anti-AFM antibody (ab152141) at 1/5000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : HeLa whole cell lysateLane 4 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.
All lanes : Anti-AFM antibody (ab152141) at 1/5000 dilutionLane 1 : NIH 3T3 whole cell lysateLane 2 : JC whole cell lysateLane 3 : BCL-1 whole cell lysateLysates/proteins at 30 µg per lane.
Immunofluorescent analysis of methanol-fixed HeLa cells labeling AFM with ab152141 at 1/500 dilution. Lower image co-stained with Hoerchst 33342.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft labeling AFM with ab152141 at 1/500 dilution.