AFM

Gene Symbol	AFM
Entrez Gene	173
Alt Symbol	ALB2, ALBA, ALF
Species	Human
Gene Type	protein-coding
Description	afamin
Other Description	alpha-Alb\|alpha-albumin
Swissprots	A8K3E1 Q4W5C5 Q32MR3 P43652
Accessions	AAC50720 AAY41051 EAX05682 P43652 AK290556 BAF83245 BC109020 AAI09021 BC109021 AAI09022 L35486 AAA68197 L35497 AAA68198 L35498 AAA68199 NM_001133 NP_001124
Function	Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier. {ECO:0000269\|PubMed:12463752, ECO:0000269\|PubMed:15952736, ECO:0000269\|PubMed:19046407}.
Subcellular Location	Secreted.
Tissue Specificity	High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level). {ECO:0000269\|PubMed:15952736}.

Afamin (G-10) - sc-373849 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

Afamin (G-10): sc-373849. Western blot analysis of Afamin expression in non-transfected (A) and human Afamin transfected (B) AFM whole cell lysates.

Afamin (H-54) - sc-135280 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Afamin (H-54): sc-135280. Western blot analysis of Afamin expression in mouse testis tissue extract.

Afamin (K-13) - sc-74311 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Afamin (S-17) - sc-74313 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-AFM antibody - ab152141 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-AFM antibody (ab152141) at 1/5000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : HeLa whole cell lysateLane 4 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-AFM antibody - ab49139 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-AFM antibody (ab49139) at 0.5 µg/mlLane 1 : Molecular weight markerLane 2 : HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50,000developed using the ECL technique

Anti-AFM antibody - ab106931 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Anti-AFM antibody (ab106931) at 0.3 µg/ml + Human Cerebellum lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-AFM / Afamin Antibody (aa22-210, Biotin) - LS-C298166 from LifeSpan Bioscience

Prices	$425.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-AFM / Afamin Antibody (aa210-403, Biotin) - LS-C298167 from LifeSpan Bioscience

Prices	$435.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-AFM / Afamin Antibody (aa311-360) - LS-C30158 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-AFM / Afamin Antibody (aa22-210, FITC) - LS-C302688 from LifeSpan Bioscience

Prices	$455.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-AFM / Afamin Antibody - LS-C306123 from LifeSpan Bioscience

Prices	$455.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Anti-AFM / Afamin Antibody (aa210-403, FITC) - LS-C302716 from LifeSpan Bioscience

Prices	$465.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-AFM / Afamin Antibody (C-Terminus) - LS-C134970 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

AFM antibody LS-C134970 Western blot of HepG2 cell lysate.

Anti-AFM / Afamin Antibody (aa211-403) - LS-C292671 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of recombinant AFM / Afamin.

Anti-AFM / Afamin Antibody (aa22-210) - LS-C292667 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-AFM / Afamin Antibody (aa210-403) - LS-C292668 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-AFM / Afamin Antibody (aa49-76) - LS-C156134 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Western blot of AFM Antibody in HepG2 cell line lysates (35 ug/lane). AFM (arrow) was detected using the purified antibody.

Anti-AFM / Afamin Antibody (aa318-330) - LS-C108133 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

AFM antibody (0.3 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Afamin Antibody - NBP2-15304 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: AFM Antibody [NBP2-15304] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE; antibody diluted at 1:5000.

Afamin Antibody (883113) - MAB8065 from Novus Biologicals

Prices	$99.00, $249.00
Sizes	25 µg, 100 µg
Host	Mouse
Clonality	Monoclonal
Clone	883113

Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Afamin Monoclonal Antibody (Catalog # MAB8065) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Afamin at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Afamin Antibody - AF8065 from Novus Biologicals

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Sheep
Clonality	Polyclonal

Afamin Antibody - H00000173-B01P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Western Blot: Afamin Antibody [H00000173-B01P] - Analysis of AFM expression in transfected 293T cell line by AFM polyclonal antibody. Lane1:AFM transfected lysate(65.89 KDa). Lane2:Non-transfected lysate.

Afamin Antibody - NBP1-89156 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry: Afamin Antibody [NBP1-89156] - Staining of human kidney shows strong cytoplasmic and extracellular material positivity in cells in tubules.

Afamin Antibody - NBP1-51973 from Novus Biologicals

Prices	$299.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Western Blot: Afamin Antibody [NBP1-51973] - Analysis of AFM in Human Cerebellum lysate (35ug protein in RIPA buffer) using NBP1-51973 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

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