Anti-Ago2 / eIF2C2 antibody - ChIP Grade
| Name | Anti-Ago2 / eIF2C2 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab32381 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP IHC-P ChIP Radioimmunoprecipitation WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Rabbit, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 350 - 450 Ago2/ eIF2C2 |
| Description | Rabbit Polyclonal |
| Gene | AGO2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Ago2 / eIF2C2 antibody - ChIP Grade (ab32381) at 2 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLysates/proteins at 20 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
IHC image of Ago2/eIF2C2 staining in human breast cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32381, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ab32381 (1/200) staining Ago2/ eIF2C2 in assynchrnous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% TritonX100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.See Abreview
RIP analysis of 368T1 murine lung cancer cells using ab32381 to bind Ago2 / eIF2C2. Cells were transfected with an empty vector (empty), a vector expressing wild-type Hmga2 (wt), a vector expressing a let-7 site mutated Hmga2 (m7), a vector expressing wild-type Hmga2 without a start codon (ATG wt), or a vector expressing a let-7 site mutated Hmga2 without a start codon (ATG m7). RIP was performed with 5 µg Ago2 / eIF2C2 or IgG antibody per 107 cells. Binding was detected using qRT-PCR.See Abreview
Product References
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Extracellular microRNAs are dynamic non-vesicular biomarkers of muscle turnover. - Extracellular microRNAs are dynamic non-vesicular biomarkers of muscle turnover.
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The HIV-1 Nef protein binds argonaute-2 and functions as a viral suppressor of - The HIV-1 Nef protein binds argonaute-2 and functions as a viral suppressor of
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MicroRNA miR-324-3p induces promoter-mediated expression of RelA gene. - MicroRNA miR-324-3p induces promoter-mediated expression of RelA gene.
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Argonaute2 complexes carry a population of circulating microRNAs independent of - Argonaute2 complexes carry a population of circulating microRNAs independent of
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